Changes between Version 24 and Version 25 of WikiStart
- Timestamp:
- 09/20/09 10:28:29 (4 years ago)
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WikiStart
v24 v25 3 3 * DNA barcoding is a tool for characterizing the species origin using a short sequence 4 4 from a standard position and agreed upon position in the genome. To be used as a DNA barcode, 5 a genome locus should vary among individuals of the same species only to a minor degree and it6 should vary among species very quickly. From a practical point of view, a barcode locus should be5 a genome locus should vary among individuals of the same species only to a minor degree and it 6 should vary among species very quickly. From a practical point of view, a barcode locus should be 7 7 flanked by two conserved regions to design PCR primers. Several manually discovered 8 barcode loci like COI, rbcL, 18S, 16S and 23S rDNA, or trnH-ps are routinely used today, but no objective function has been described to measure their 8 barcode loci like COI, rbcL, 18S, 16S and 23S rDNA, or trnH-ps are routinely used today, but no 9 objective function has been described to measure their 9 10 quality in terms of universality (barcode coverage, Bc ) or in terms of taxonomical discrimination 10 11 capacity (barcode specificity, Bs ).
